
immusmol/组胺释放试剂盒(附件)-全血样本/BA-E-1100
市场价:
¥1400.00
美元价:
700.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Product information | Key steps | References | Technical support | Reviews |
Product overview
Product name | Histamine release kit - Whole blood samples |
Description | Supplementary kit to in vitro diagnostics enzyme immunoassay (ELISA) for the quantitative determination of histamine release in whole blood samples. |
Labels | IVD, CE |
Format | 96-well plate |
Samples | 500 µl whole blood |
Cross specie-reactivity | React with all species |
Standard range | 0 / 0.5 - 50 ng/ml |
Sensitivity | 0.2 ng/ml |
Specificity | No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin |
Assay time | 1h30 sample preparation + ELISA overnight |
Storage | Store at 2-8°C for up to 6 months. |
Datasheets | Instructions for use , Material safety datasheet |
Key steps
Sample collection & storage | 24-hours heparinized whole blood stored at 20-25°C. |
Sample preparation | Sample acylation (1h30) |
ELISA | Histamine antiserum incubation (3H or overnight), revelation and read steps (1h). |
Detailed protocol | Download instructions for use |
References
- Chirumbolo S. et al. Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets. Clin Mol Allergy. 2010 Sep 17;8:13.
- Martin SK et al. Influence of Dietary Histidine on Basophil Release, Circulating Concentration, and Urinary Excretion of Histamine in Domestic Felines. Intern J Appl Res Vet Med. 2012. 10: 289-299
- McCord JL et al. H2-receptor-mediated vasodilation contributes to postexercise hypotension. J Appl Physiol (1985). 2006 Jan;100(1):67-75
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