| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Serotonin ELISA kit - Serum, Urine, Platelets - Fast Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of 5-hydroxytryptamine (5-HT, Serotonin) in serum, urine or platelets. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | 25 µl serum, urine, platelets |
| Cross-species reactivity | React with all species |
| Standard range | 0 / 15 - 2 500 ng/ml |
| Sensitivity | 5 ng/ml |
| Specificity | No significant cross-reactivity was observed with Serotonin analogs such as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine, Histidine, Tyramine and 5-Hydroxytryptophan. |
| Assay time | 1h15 |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | Serum: Do not use haemolytic and especially lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C. |
| Sample preparation | Sample acylation (15min) |
| ELISA | Serotonin antiserum incubation (30min), revelation and read steps (30min). |
| Detailed protocol | Download instructions for use |
References
- Product citation: Ishii et al. Dietary Supplementation with Lysine and Threonine Modulates the Performance and Plasma Metabolites of Broiler Chicken. Animals (Basel). 2019 August 26
- Product citation: Lee et al. The effects of acupuncture on serotonin metabolism.
- Sanner JE, Frazier L, Udtha M. Effects of delayed laboratory processing on platelet serotonin levels. Biol Res Nurs. 2013 Jan;15(1):13-6.
- Ciuclan L et al. A novel murine model of severe pulmonary arterial hypertension. Am J Respir Crit Care Med. 2011 Nov 15;184(10):1171-82.
- El-Ansary AK, Bacha AB, Ayahdi LY. Relationship between chronic lead toxicity and plasma neurotransmitters in autistic patients from Saudi Arabia. Clin Biochem. 2011 Sep;44(13):1116-20.
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